A Study on Applying Slide-Free Label-Free Harmonic Generation Microscopy For Noninvasive Assessment of Melasma Treatments With Histopathological Parameters

Melasma, which is thought to be associated with hyperactivation of melanocytes, a common hyperpigmentary skin disorder. To treat this disorder, dermatologists can benefit from in vivo information cellular morphometrics evaluate pathologies melasma and effectiveness treatments. acquire useful information, we applied the slide-free label-free harmonic generation microscopy (HGM) retrieve real-time HGM images subsequent critical histopathological parameters. These quantitative parameters included melanin mass density (MMD), melanocyte dendricity score (MDS), melanophage (MPD), thickness dermal papilla zone (DPZ). The statistical results 33 recruited Asian female patients showed that MMD, MDS, MPD lesions were significantly higher than those surrounding normal skin; by contrast, DPZ was lower. After treatment, MPD, restored toward level; however, observed no significant change MDS. Our study indicates these are able serve as clinical indices characteristics